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fluorescein conjugated avidin d  (Vector Laboratories)


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    Structured Review

    Vector Laboratories fluorescein conjugated avidin d
    Fluorescein Conjugated Avidin D, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1558 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fluorescein+conjugated+avidin+d/Avidin+D/pmc13081366-442-17-28
    Average 96 stars, based on 1558 article reviews
    fluorescein conjugated avidin d - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Avidin-Biotin Assay:

    Article Title: Tribolium madens satellitome reveals a network of highly abundant satellite DNAs in megabase-sized regions hallmarked by macro-dyad symmetries
    Article Snippet: .. Cy3-labeled probes were detected directly after posthybridization washes, while detection of biotin-labeled probes was achieved using a fluorescein-conjugated avidin D (FITC-avidin D) and biotinylated anti-avidin D amplification system (Vector Laboratories, USA). .. Signal enhancement was performed through a three-step sequential incubation using the following reagent dilutions: 1:500 FITC-avidin D, 1:100 biotinylated anti-avidin D, and 1:2000 FITC-avidin D. Slides were subsequently counterstained with 4′,6-diamidino-2-phenylindole (DAPI) for 15 min, air-dried, and mounted in Mowiol 4–88 mounting medium (Sigma-Aldrich, USA).

    Article Title: Myoscaffolds reveal laminin scarring is detrimental for stem cell function while sarcospan induces compensatory fibrosis
    Article Snippet: Primary antibodies were detected by biotinylated anti-rabbit (BA-1000; 1:500; Vector Laboratories). .. Fluorescein-conjugated avidin D (A-2001; 1:500; Vector Laboratories) was used to detect secondary antibodies. .. Laminin alpha-2 was used specifically for costaining and was detected by Alexa Fluor 594 Donkey anti-rat (AB150156; 1:200; Abcam).

    Article Title: Mast cells promote choroidal neovascularization in a model of age-related macular degeneration
    Article Snippet: .. The RPE/choroid complex was then stained with 1:100 FITC-labeled isolectin (Vector Lab; FL-1201), rhodamine-labeled isolectin (Vector Lab; #RL-1102), 1:200 rhodamine phalloidin (Cedarlane Laboratories), 1:100 tryptase (ThermoFisher: #PA5-119480), or 1:400 fluorescein-conjugated avidin D (Vector Lab; #A-2001-5) overnight. ..

    Article Title: Multi-omics analysis of sarcospan overexpression in mdx skeletal muscle reveals compensatory remodeling of cytoskeleton-matrix interactions that promote mechanotransduction pathways
    Article Snippet: Primary antibodies were detected by biotinylated anti-rabbit (BA-1000; 1:500; Vector Laboratories, Newark, CA, USA). .. Fluorescein-conjugated avidin D (A-2001; 1:500; Vector Laboratories) was used to detect secondary antibodies. .. Sections were mounted in Vectashield (Vector Laboratories, Newark, CA, USA), and imaging was performed using a Zeiss Axio Imager M2 (Carl Zeiss Inc., Thornwood, NY, USA) with a Hamamatsu ORCA-Flash 4.0 V3 digital complementary metal oxide semiconductor camera and a plan-Apochromat 20×/0.8 M27 objective.

    Article Title: Myoscaffolds reveal laminin scarring is detrimental for stem cell function while sarcospan induces compensatory fibrosis.
    Article Snippet: Primary antibodies were detected by biotinylated anti-rabbit (BA-1000; 1:500; Vector Laboratories). .. Fluorescein-conjugated avidin D (A-2001; 1:500; Vector Laboratories) was used to detect secondary antibodies. .. Laminin alpha-2 was used specifically for costaining and was detected by Alexa Fluor 594 Donkey anti-rat (AB150156; 1:200; Abcam).

    Article Title: Tribolium madens satellitome reveals a network of highly abundant satellite DNAs in megabase-sized regions hallmarked by macro-dyad symmetries.
    Article Snippet: For the TmSat3 probe, considering TmSat3 monomer length of 1264 bp, we used four different primer pairs to bridge the entire monomer sequence, using the TmSat3 monomer fragment cloned into the plasmid vector pUC18 as a template. .. Cy3-labeled probes were detected directly after posthybridization washes, while detection of biotin-labeled probes was achieved using a fluorescein-conjugated avidin D (FITC-avidin D) and biotinylated anti-avidin D amplification system (Vector Laboratories, USA). .. The 500 bp TmSat2-specific fragment, cloned into the plasmid vector pUC18, was biotin-labeled by nick translation using the Nick Translation Kit (Roche, Switzerland).

    Article Title: The Art of Contemporary Drug Discovery: Developing Novel Small Molecule Therapeutics For Neurological and Muscular Disorders
    Article Snippet: .. Bound antibodies were visualized by incubation with Fluorescein-conjugated Avidin D (Vector Laboratories). .. Slides were wet mounted in Vectashield with or without DAPI (Vector Laboratories) before analysis by microscopy on Zeiss Axio Observer 7 or Axio Imager M2 (Carl Zeiss).

    Amplification:

    Article Title: Tribolium madens satellitome reveals a network of highly abundant satellite DNAs in megabase-sized regions hallmarked by macro-dyad symmetries
    Article Snippet: .. Cy3-labeled probes were detected directly after posthybridization washes, while detection of biotin-labeled probes was achieved using a fluorescein-conjugated avidin D (FITC-avidin D) and biotinylated anti-avidin D amplification system (Vector Laboratories, USA). .. Signal enhancement was performed through a three-step sequential incubation using the following reagent dilutions: 1:500 FITC-avidin D, 1:100 biotinylated anti-avidin D, and 1:2000 FITC-avidin D. Slides were subsequently counterstained with 4′,6-diamidino-2-phenylindole (DAPI) for 15 min, air-dried, and mounted in Mowiol 4–88 mounting medium (Sigma-Aldrich, USA).

    Article Title: Tribolium madens satellitome reveals a network of highly abundant satellite DNAs in megabase-sized regions hallmarked by macro-dyad symmetries.
    Article Snippet: For the TmSat3 probe, considering TmSat3 monomer length of 1264 bp, we used four different primer pairs to bridge the entire monomer sequence, using the TmSat3 monomer fragment cloned into the plasmid vector pUC18 as a template. .. Cy3-labeled probes were detected directly after posthybridization washes, while detection of biotin-labeled probes was achieved using a fluorescein-conjugated avidin D (FITC-avidin D) and biotinylated anti-avidin D amplification system (Vector Laboratories, USA). .. The 500 bp TmSat2-specific fragment, cloned into the plasmid vector pUC18, was biotin-labeled by nick translation using the Nick Translation Kit (Roche, Switzerland).

    other:

    Article Title: Sarcospan protects against LGMD R5 via remodeling of the sarcoglycan complex composition in dystrophic mice
    Article Snippet: Diluent at 4°C overnight: dystrophin (MANDYS8; D8168, 1:200; Sigma-Aldrich), utrophinM3 (MANCHO3 84A, 1:5; Developmental Studies Hybridoma Bank), a-sarcoglycan (NCL-L-a-SARC, 1:100; Leica Biosystems), b-sarcoglycan (NCL-L-b-SARC, 1:20; Leica Biosystems), g-sarcoglycan (GTX117176, 1:4000; GeneTex), dsarcoglycan (ab137101, 1:100; Abcam), e-sarcoglycan (2407-1-AP, 1:50; Thermo Fisher Scientific), z-sarcoglycan (NBP2-48536, 1:50; Novus Biologicals), b-dystroglycan (NCL-L-bSARC, 1:20; Leica Biosystems), a-dystroglycan (DGIIH6 C4s, 1:20; Developmental Studies Hybridoma Bank), b1D integrin (MAB1900, 1:30; Millipore), laminin (L9393; 1:200; SigmaAldrich), spectrin (PA5-52970, 1:200; Thermo Fisher Scientific), eMHC (F1.652, 1:25; Developmental Studies Hybridoma Bank), SSPN E2 (sc-393187; 1:50; Santa Cruz Biotechnology), type I collagen (CL50151AP-1; 1:250; Cedarlane Labs), and laminin alpha-2 (AB11576; 1:150; Abcam).

    Staining:

    Article Title: Mast cells promote choroidal neovascularization in a model of age-related macular degeneration
    Article Snippet: .. The RPE/choroid complex was then stained with 1:100 FITC-labeled isolectin (Vector Lab; FL-1201), rhodamine-labeled isolectin (Vector Lab; #RL-1102), 1:200 rhodamine phalloidin (Cedarlane Laboratories), 1:100 tryptase (ThermoFisher: #PA5-119480), or 1:400 fluorescein-conjugated avidin D (Vector Lab; #A-2001-5) overnight. ..

    Incubation:

    Article Title: The Art of Contemporary Drug Discovery: Developing Novel Small Molecule Therapeutics For Neurological and Muscular Disorders
    Article Snippet: .. Bound antibodies were visualized by incubation with Fluorescein-conjugated Avidin D (Vector Laboratories). .. Slides were wet mounted in Vectashield with or without DAPI (Vector Laboratories) before analysis by microscopy on Zeiss Axio Observer 7 or Axio Imager M2 (Carl Zeiss).



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